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DiagnosticPathology & Laboratory MedicineHighly effective Success Rate

Immunohistochemistry

Pathology & Laboratory Medicine · Diagnostic

2-5 days turnaround

Duration

Outpatient

Setting

There is no recovery involved as this is purely a laboratory analysis. Results typically take 2-5 days depending on the number of markers tested and are incorporated into the final pathology report used to guide treatment.

Recovery

₹2,500+

Cost

📖About Immunohistochemistry

Immunohistochemistry (IHC) is a specialised laboratory technique that uses antibodies to detect specific proteins within tissue samples, helping pathologists precisely classify tumours and other diseases beyond what standard microscopy alone can reveal. It is especially important in cancer diagnosis, where identifying specific protein markers can determine the exact tumour type, its origin, and whether it is likely to respond to targeted therapies such as hormone or immunotherapy treatments. The technique works by applying antibodies that bind to particular proteins in the tissue, which are then visualised with a colour-producing stain under the microscope. IHC has become an essential complement to routine histopathology in modern cancer diagnosis.

Duration

2-5 days turnaround

Setting

Outpatient

Recovery

There is no recovery involved as this is purely a laboratory analysis. Results typically take 2-5 days depending on the number of markers tested and are incorporated into the final pathology report used to guide treatment.

Cost Range

₹2,500 – ₹8,000 per marker

Specialty

Pathology & Laboratory Medicine

Success Rate

Highly effective

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📋Indications — Who Needs This Treatment?

  • 1Determining the origin of a metastatic tumour of unknown primary, subtyping breast cancer through hormone receptor and HER2 status, distinguishing between similar-appearing tumour types, confirming lymphoma subtype, guiding decisions on targeted cancer therapies
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🔬How the Treatment is Performed

  • 1Thin sections of the previously processed tissue sample are mounted on slides and treated with specific antibodies designed to bind to particular proteins of interest, followed by a secondary detection system that produces a visible colour reaction wherever the antibody has bound. The pathologist then examines the stained slides under the microscope, assessing the pattern, intensity, and proportion of cells showing a positive reaction to help characterise the tissue or tumour precisely.

📝Pre-Treatment Preparation

  • 1Consult a Pathology & Laboratory Medicine specialist to confirm suitability for Immunohistochemistry
  • 2Complete all pre-treatment investigations as directed — blood tests, imaging, ECG, or specialist clearance
  • 3Disclose all current medications, supplements, and allergies to your treating physician
  • 4Fast (nil by mouth) for 6–8 hours if the treatment involves anaesthesia or sedation
  • 5Arrange for a responsible adult to accompany you, especially for procedures requiring sedation
  • 6Follow all specific pre-treatment instructions issued by your care team regarding diet or medication changes
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⚠️Possible Side Effects & Risks

  • 1No direct risk to the patient since this is a laboratory test performed on already-collected tissue, occasional need for additional markers if initial results are inconclusive, added cost and turnaround time compared to standard staining

🩹Recovery & Aftercare

  • 1There is no recovery involved as this is purely a laboratory analysis. Results typically take 2-5 days depending on the number of markers tested and are incorporated into the final pathology report used to guide treatment.

Treatment Journey — What to Expect

  1. Before Treatment

    Pre-treatment workup, consent signing, and preparation as instructed by your care team. Baseline tests and imaging completed.

  2. During Treatment

    Immunohistochemistry is administered over 2-5 days turnaround. Vital signs monitored. Your specialist manages any immediate side effects.

  3. Immediate Post-Treatment

    Recovery observation, assessment of initial response, discharge instructions provided. Follow-up appointment scheduled.

  4. Long-term Recovery (There is no recovery involved as this is purely a laboratory analysis. Results typically take 2-5 days depending on the number of markers tested and are incorporated into the final pathology report used to guide treatment.)

    Return to normal activities as tolerated. Monitoring for treatment response and side effects. Ongoing support from your specialist.

Immunohistochemistry Cost in India (2024–25)

ComponentGovt / BudgetPrivate
Specialist Consultation₹300 – ₹800₹1,000 – ₹3,000
Procedure / Session Cost₹2,000 – ₹10,000₹10,000 – ₹50,000
Medications₹500 – ₹3,000₹2,000 – ₹15,000
Follow-up & Monitoring₹300 – ₹1,000₹1,000 – ₹5,000
Rehabilitation / Aftercare₹1,000 – ₹5,000₹5,000 – ₹25,000

* Estimates for India 2024–25. CGHS / Ayushman Bharat may cover eligible treatments. Individual costs vary by hospital, city, and complexity.

Treatment Outcomes

Immunohistochemistry has a documented success rate of Highly effective when performed by qualified specialists at accredited facilities. Individual outcomes vary based on disease stage, patient health, and adherence to the treatment plan and aftercare instructions.

Frequently Asked Questions

What is Immunohistochemistry?+

Immunohistochemistry (IHC) is a specialised laboratory technique that uses antibodies to detect specific proteins within tissue samples, helping pathologists precisely classify tumours and other diseases beyond what standard microscopy alone can reveal. It is especially important in cancer diagnosis, where identifying specific protein markers can determine the exact tumour type, its origin, and whether it is likely to respond to targeted therapies such as hormone or immunotherapy treatments. The technique works by applying antibodies that bind to particular proteins in the tissue, which are then visualised with a colour-producing stain under the microscope. IHC has become an essential complement to routine histopathology in modern cancer diagnosis.

Who is a candidate for Immunohistochemistry?+

Immunohistochemistry is recommended for patients diagnosed with specific conditions where other approaches have been insufficient. Your Pathology & Laboratory Medicine specialist will assess your suitability based on your diagnosis, health history, and treatment goals.

How long does Immunohistochemistry take?+

The typical duration is 2-5 days turnaround. This may vary based on the complexity of your case and individual response to treatment.

What is the recovery time after Immunohistochemistry?+

Recovery typically takes There is no recovery involved as this is purely a laboratory analysis. Results typically take 2-5 days depending on the number of markers tested and are incorporated into the final pathology report used to guide treatment.. Your care team will provide specific instructions based on your response and overall health.

What are the risks or side effects of Immunohistochemistry?+

Side effects vary by individual and are detailed in the section above. Your specialist will discuss all risks and benefits with you before treatment begins. Serious adverse effects are uncommon but are part of the informed consent process.

What is the success rate of Immunohistochemistry?+

Immunohistochemistry has a documented success rate of Highly effective at accredited facilities. Outcomes depend on disease stage, patient health, and adherence to the treatment plan and aftercare instructions.

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Quick Cost Estimate

Govt / Budget Hospital₹2,500 approx.
Private Hospital₹2,500 – ₹8,000 per marker
Online / Home Care₹199 – ₹2,000
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⚕️ Medical Disclaimer

This page is for informational purposes only. Always consult a qualified specialist before deciding on any treatment. In emergencies, call 112.